Before the optimization will be performed you need to select the parameters to fit. To do this open the tab with the diagram parameters (variables), select the parameters (variables) which you want to fit, and click the up arrow. The list of the selected parameters appear in the table located above.

In order to remove unnecessary parameters from the fitting parameters set, select them in the table and click the down arrow. If you want to change the initial values of some parameters (variables), enter them in the column "Initial value" of the table with the diagram parameters (variables). The values of the relevant parameters (variables) are automatically changed in the fitting parameters table.
Fitting parameters can be specified as local or global to work with experimental data obtained for the different cell lines. For more details see Experimental data chapter.